Human Factor XI ELISA Kit
Factor XI is a disulfide linked two-chain glycoprotein zymogen and is the precursor of the coagulation enzyme Factor XIa. Factor XI consists of two identical monomers and circulates in plasma in complex with kininogen. Factor XI is activated by Factor XIIa and converts Factor IX to Factor IXa during the intrinsic pathway of the coagulation cascade.
This human coagulation Factor XI antigen assay is intended for the quantitative determination of total Factor XI antigen in human plasma. The assay measures total human Factor XI in the 0.01-10 ng/ml range. Samples giving human Factor XI levels above 10ng/ml should be diluted in blocking buffer before use. A 1:2,000-1:10,000 dilution for normal human plasma is suggested for best results. Strip well format. Reagents for up to 96 tests.
Human Factor XI will bind to the capture antibody coated on the microtiter plate. Both Factor XI and XIa will react with the antibody on the plate. After appropriate washing steps, biotinylated primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with horseradish peroxidase conjugated streptavidin. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human Factor XI. Color development is proportional to the concentration of Factor XI in the samples.
Detection Target: Factor XI
Range: 0.01 - 10 ng/ml
Sensitivity: 0.012 ng/ml
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