Human Factor VIII Total Antigen ELISA Kit
This human coagulation Factor VIII antigen assay is intended for the quantitative determination of total Factor VIII antigen in human plasma.
Factor VIII (aka Factor VIII:C or Antihemophilic Globulin) is a glycoprotein zymogen that circulates in a stabilized non-covalent complex with von Willebrand Factor (vWF) 1. Following activation by thrombin or Factor Xa, Factor VIIIa dissociates from vWF and catalyzes the activation of Factor X by Factor IXa in the amplification phase of coagulation 2. Factor VIIIa activity is quickly decreased by spontaneous dissociation and proteolytic degradation by activated Protein C, Factor Xa and Factor IXa 3. Hemophilia A is caused by mutations in the Factor VIII gene; a majority of patients have decreased Factor VIII plasma levels while 5% of patients have normal levels of nonfunctioning protein 4.
The Factor VIII level in the human plasma standard provided is calibrated against a secondary standard that is referenced to the WHO or ISTH International Standard.
The Simultaneous Human FVIII/vWF Purification and Virus Inactivation Combined in Chromatographic Column
Sergiy P Havryliuk, Ievgenia M Krasnobryzha, Olena S Havryliuk and Georgii L Volkov - Journal of Biomolecular Research & Therapeutics, Aug 14 2017
... The proteins quantity were measured by the standard methods with Human Factor VIII total antigen ELISA kit and Human vWF ELISA kit (Innovative Research, Novi, USA) and chromogenic kits from Chromogenix AB (Mldland, Sweden) ...