Human Factor VIII Total Antigen ELISA Kit
Factor VIII (aka Factor VIII:C or Antihemophilic Globulin) is a glycoprotein zymogen that circulates in a stabilized non-covalent complex with von Willebrand Factor (vWF). Following activation by thrombin or Factor Xa, Factor VIIIa dissociates from vWF and catalyzes the activation of Factor X by Factor IXa in the amplification phase of coagulation. Factor VIIIa activity is quickly decreased by spontaneous dissociation and proteolytic degradation by activated Protein C, Factor Xa and Factor IXa. Hemophilia A is caused by mutations in the Factor VIII gene; a majority of patients have decreased Factor VIII plasma levels while 5% of patients have normal levels of nonfunctioning protein.
This human coagulation Factor VIII antigen assay is intended for the quantitative determination of total Factor VIII antigen in human plasma. The assay measures Factor VIII antigen in the 0.0016 - 0.84 IU/ml range. 1:50 and 1:100 dilutions for normal plasma, or 1:4 and 1:8 dilutions for Haemophiliac plasma, are suggested for best results. Strip well format. Reagents for up to 96 tests.
Human Factor VIII will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled anti-human Factor VIII primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human plasma. Color development is proportional to the concentration of Factor VIII in the samples.
Detection Target: Factor VIII
Range: 0.0016 - 0.84 IU/ml
Sensitivity: 0.00042 IU/ml
Disclaimer: This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herein are offered for informational purposes only to be used solely for your consideration, investigation, and verification.
The Simultaneous Human FVIII/vWF Purification and Virus Inactivation Combined in Chromatographic Column
Sergiy P Havryliuk, Ievgenia M Krasnobryzha, Olena S Havryliuk and Georgii L Volkov - Journal of Biomolecular Research & Therapeutics, Aug 14 2017
... The proteins quantity were measured by the standard methods with Human Factor VIII total antigen ELISA kit and Human vWF ELISA kit (Innovative Research, Novi, USA) and chromogenic kits from Chromogenix AB (Mldland, Sweden) ...