Human Factor IX Total ELISA Kit
Factor IX (aka Christmas Factor) is a single-chain, 415 amino acid glycoprotein zymogen. Factor IX is activated by either Factor XIa or the Factor VIIa complex. Factor IXa converts Factor X to Factor Xa during the intrinsic pathway of the coagulation cascade. Factor IX is used to treat patients with hemophilia B, an X-linked bleeding disorder.
This human coagulation Factor IX antigen assay is intended for the quantitative determination of total Factor IX antigen in human plasma. The assay measures Factor IX antigen in the 0.1 - 100 ng/ml range. If the unknown is thought to have high Factor IX levels, dilutions may be made in blocking buffer. A 1:1,000 to 1:4,000 dilution for normal plasma is suggested for best results. Strip well format. Reagents for up to 96 tests.
Human Factor IX will bind to the affinity purified capture antibody coated on the microtiter plate. Factor IX and IXa will react with the antibody on the plate. After appropriate washing steps, polyclonal anti-human Factor IX primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody, which is proportional to the total Factor IX present in the samples, is reacted with the secondary antibody. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human Factor IX. Color development is proportional to the concentration of Factor IX in the samples.
Detection Target: Factor IX
Range: 0.1 - 100 ng/ml
Sensitivity: 0.044 ng/ml
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