Human Factor H ELISA Kit
The Human Complement Factor H (FH) ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of human FH in urine, saliva, milk, plasma, serum, and cell culture supernatant samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures FH in less than 4 hours. A polyclonal antibody specific for FH has been pre-coated onto a 96-well microplate with removable strips. Human FH in standards and samples is sandwiched by the immobilized antibody and the biotinylated polyclonal antibody specific for human FH, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.