Human Complement Factor D ELISA KitThe Human Complement Factor D (FD) ELISA kit is designed for detection of human FD in plasma, serum, saliva, milk, and cell culture samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures FD in less than 4 hours. A polyclonal antibody specific for FD has been pre-coated onto a 96-well microplate with removable strips. Human FD in standards and samples is sandwiched by the immobilized antibody and the biotinylated polyclonal antibody specific for human FD, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.