Human Complement Factor I ELISA KitThe Complement Factor I ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of complement factor I in plasma, serum, milk, saliva, and cell culture supernatant. This assay employs a quantitative competitive enzyme immunoassay technique that measures factor I in less than 3 hours. A polyclonal antibody specific for factor I has been pre-coated onto a 96-well microplate with removable strips. Factor I in standards and samples is competed with a biotinylated factor I sandwiched by the immobilized antibody and streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.