Human Complement C5 ELISA Kit
Human Complement C5 ELISA Kit is designed for detection of C5 in human plasma, serum, saliva, milk, and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures C5 in less than 4 hours. A polyclonal antibody specific for C5 has been pre-coated onto a microplate. C5 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for C5, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
Human Complement Component 5 (C5) is the fifth component of the complement system. C5 has a molecular weight of about 195 kDa and matures into heterodimers with - and -chains of 120 kDa and 75 kDa,respectively (1). Upon complement activation, C5 is cleaved into a small C5a and larger C5b polypeptides by C5 convertase. The potent pro-inflammatory anaphylatoxin C5a binds to receptors C5aR and C5L2 to initiate acute inflammatory responses (2 - 3). The C5a-mediated early pro-inflammatory responses include sepsis, systemic lupus erythamatosis, and cerebral malaria (4 - 6). The larger C5b interact with complement components C6, C7, C8, and C9 to form a membrane attack complex C5b-9 which is involved in cell apoptosis, cell activation, and production of proinflammatory mediators (7). C5 deficiency is associated with severe infantile dermatitis Leiner's disease (8).