Human Ceruloplasmin ELISA Kit
Ceruloplasmin (aka Ferroxidase I) is a 132kDa 1,046 amino acid glycoprotein which carries 95% of serum copper by binding 6 cupric ions per molecule. Levels are decreased in Wilson's Disease (hepatolenticular degeneration) and heritable aceruloplasminemia leading to iron accumulation in the liver or brain from impaired iron homeostasis.
This human ceruloplasmin antigen assay is intended for the quantitative determination of total ceruloplasmin antigen in human plasma, serum, urine, milk, saliva and cell culture samples. The assay measures total human ceruloplasmin in the 1 - 1,000 ng/ml range. If the unknown is thought to have high ceruloplasmin levels, dilutions may be made in blocking buffer. A 1:10,000-1:100,000 dilution for normal plasma or serum and 1:10-1:50 dilution for breast milk is suggested for best results. Saliva and urine samples should be applied directly to the plate for best results. Strip well format. Reagents for up to 96 tests.
Human ceruloplasmin will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled anti human ceruloplasmin primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human ceruloplasmin. Color development is proportional to the concentration of total ceruloplasmin in the samples.
Detection Target: Ceruloplasmin
Range: 1 - 1000 ng/ml
Sensitivity: 0.333 ng/ml
Disclaimer: This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herein are offered for informational purposes only to be used solely for your consideration, investigation, and verification.