Human C-Reactive Protein ELISA Kit (contain negative plasma control & WHO reference standard calibrated positive control)The Human C-Reactive Protein ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of human CRP in plasma, serum, milk, urine, and cell culture supernatant samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures CRP in less than 4 hours. A murine antibody specific for CRP has been pre-coated onto a 96-well microplate with removable strips. CRP in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for CRP, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.