Human Apolipoprotein B ELISA Kit
ApoB is the primary protein constituent of low density lipoprotein, very-low density lipoprotein, and chylomicrons. ApoB directs cholesterol and triglyceride containing particles to tissues by ApoB receptor binding and internalization. The ratio of ApoB to Apolipoprotein A1 (ApoA1), the major protein component of high density lipoprotein, is an effective predictor of cardiovascular disease.
This human Apolipoprotein B (ApoB) antigen assay is intended for the quantitative determination of total ApoB in human plasma, serum, urine & other biological fluids. The assay measures ApoB antigen in the 5 - 5,000 ng/ml range. Samples giving human ApoB levels above 5,000 ng/ml should be diluted in diluent before use. A 1:20,000 to 1:80,000 dilution for normal human plasma is suggested for best results. Strip well format. Reagents for up to 96 tests.
Human ApoB will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, monoclonal anti-human ApoB primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with the peroxidase conjugated secondary antibody. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human ApoB. The amount of color development is proportional to the concentration of total ApoB antigen in the sample.
Detection Target: Apolipoprotein B
Range: 5 - 5,000 ng/ml
Sensitivity: 1.5 ng/ml
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