Human Apolipoprotein Assay and ELISA Kits

Human Apolipoprotein A1 ELISA Kit

Catalog No.: IRAPKT020


Category: Assay and ELISA Kits

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Product Description

Human Apolipoprotein A1 ELISA Kit

ApoA1 is the major protein constituent of high density lipoprotein which is the densest of the lipoprotein aggregates. ApoA1 participates in the reverse transport of cholesterol from tissues to the liver and is a cofactor for lecithin cholesterol acyl tranferase. The ratio of ApoA1 to Apolipoprotein B (ApoB), the primary component of low density lipoprotein, is an effective predictor of cardiovascular disease.

This human apolipoprotein A1 (ApoA1) antigen assay is intended for the quantitative determination of total ApoA1 in human plasma, serum, urine & other biological fluids. The assay measures ApoA1 antigen in the 5 - 5,000 ng/ml range. Samples giving human ApoA1 levels above 5,000 ng/ml should be diluted in diluent before use. A 1:40,000 to 1:80,000 dilution for normal human plasma is suggested for best results. Strip well format. Reagents for up to 96 tests.

Human ApoA1 will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, monoclonal anti-human ApoA1 primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with the peroxidase conjugated secondary antibody. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human ApoA1. The amount of color development is proportional to the concentration of total ApoA1 antigen in the sample.

Detection Target: Apolipoprotein A1
Reactivity: Human
Range: 5 - 5,000 ng/ml
Sensitivity: 4.9 ng/ml

Disclaimer: This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herein are offered for informational purposes only to be used solely for your consideration, investigation, and verification.

Triglyceride Recrystallized Phytosterols in Fat-Free Milk Improve Lipoprotein Profiles More Than Unmodified Free Phytosterols in Hypercholesterolemic Men and ...

LJ Kunces, LK Cusack, BR Kupchak, BM Volk... - Journal of the American ..., 2013
...below 11.2%. Plasma apolipoprotein A1 (apoA1) and apoB were analyzed in duplicate by enzyme-linked immunosorbent assay (Innovative Research, Novi, MI) after 200-and 20,000-fold dilutions, respectively. The apoA1 assay...

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Human Apolipoprotein A1 ELISA Kit

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