Human Apolipoprotein AII ELISA Kit (High Sensitivity Format)The Human Apolipoprotein A-II ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of human apoA-II in plasma, serum, and cell culture samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures human apoA-II in less than 4 hours. A polyclonal antibody specific for human apoA-II has been pre-coated onto a 96-well microplate with removable strips. ApoA-II in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for human apoA-II, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.