Human Alpha 2 Antiplasmin Total Antigen ELISA Kit
alpha2-antiplasmin is the major circulating inhibitor of plasmin. It plays a role in the regulation of intravascular fibrinolysis. Decreased levels of alpha2-antiplasmin may play an important role in the increased capacity of the fibrinolytic function and may be beneficial in the treatment of thrombotic diseases, acute pulmonary embolism, and hepatic repair.
This human alpha2-antiplasmin assay is for the quantitative determination of total alpha2-antiplasmin in human plasma. The assay measures alpha2-antiplasmin antigen in the 0.1-100 ng/ml range. If the unknown is thought to have high alpha2-antiplasmin levels, dilutions may be made in blocking buffer. A 1:50,000 dilution for normal human plasma is suggested for best results. Strip well format. Reagents for up to 96 tests.
Total human alpha2-antiplasmin will bind to the affinity purified capture antibody coated on the microtiter plate. Complexed and free alpha2-antiplasmin will react with the antibody on the plate. After appropriate washing steps, polyclonal anti-human alpha2-antiplasmin primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody, which is proportional to the total alpha2-antiplasmin present in the samples, is reacted with horseradish peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human alpha2- antiplasmin. Color development is proportional to the concentration of alpha2-antiplasmin in the samples.
Detection Target: Alpha-2-antiplasmin
Range: 0.1 - 100 ng/ml
Sensitivity: 0.028 ng/ml
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