Human Alpha-2 Macroglobulin ELISA Kit
Human Alpha-2 Macroglobulin ELISA Kit is for the quantitative determination of A2M in human plasma and other biological fluids. Human A2M will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, polyclonal anti-human A2M primary antibody binds to the A2M. Excess antibody is washed away and bound polyclonal antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. Following an additional washing step, TMB substrate is used for color development at 450nm. The amount of color development is directly proportional to the concentration of total A2M in the sample.