Human Alpha-2 Macroglobulin ELISA Kit
A2M is a circulating 720 kDa homotetramer expressed in the liver which captures a wide range of plasma proteinases including plasmin and thrombin. Each monomer contains an internal thiol ester, a transglutaminase reactive site, zinc and receptor binding sites, and a bait region which when cleaved induces a conformational change trapping the proteinase. Serum A2M levels are decreased in acute pancreatitis and increased in chronic liver disease and nephrotic syndrome.
This human alpha-2-macroglobulin (A2M) assay is for the quantitative determination of A2M in human plasma and other biological fluids. The assay measures A2M in the 0.5 - 250 ng/ml range. If the unknown is thought to have high A2M levels, dilutions may be made in blocking buffer. A 1:100,000 to 1:400,000 dilution for normal human plasma is suggested for best results. Strip well format. Reagents for up to 96 tests.
Human A2M will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, biotinylated primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with horseradish peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450nm. The amount of color development is directly proportional to the concentration of total A2M in the sample.
Detection Target: Alpha-2-macroglobulin
Range: 0.5 - 250 ng/ml
Sensitivity: 0.123 ng/ml
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