Human Albumin ELISA Kit
Albumin is a water-soluble protein with considerable structural stability which makes up 60% of the total protein of plasma. It functions as a carrier of hormones, enzymes, fatty acids, metal ions, and medicinal products.
This human albumin antigen assay is intended for the quantitative determination of total albumin in human plasma, serum, urine & other biological fluids. The assay measures albumin antigen in the 0.5 - 200 ng/ml range. Samples giving human albumin levels above 200 ng/ml should be diluted in diluent before use. A 1:4,000,000 to 1:16,000,000 dilution for normal human plasma and 1:1,000 dilution for normal human urine is suggested for best results. BSA concentrations of greater than or equal to 0.1ug/ml in buffers will interfere with the assay and cause high background levels. Strip well format. Reagents for up to 96 tests.
Human albumin will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, polyclonal anti-human albumin primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody, which is proportional to the total albumin present in the samples, is reacted with the secondary antibody. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human albumin. The amount of color development is proportional to the concentration of albumin in the sample.
Detection Target: Albumin
Range: 0.5 - 200 ng/ml
Sensitivity: 0.23 ng/ml
Disclaimer: This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herein are offered for informational purposes only to be used solely for your consideration, investigation, and verification.
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