Horse Serum Amyloid A ELISA Kit
Horse Serum Amyloid A ELISA Kit is a quantitative ELISA-based test kit for detecting serum amyloid A in horse biological samples.
In this assay the Serum Amyloid A present in samples reacts with the anti-Serum Amyloid A antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, the Detection Antibody and the HRP-Streptavidin conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound SAA. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3,5,5-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of SAA in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of SAA in the test sample. The quantity of SAA in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.