x
Horse IgA Assay and ELISA Kits

Horse IgA ELISA Kit

Catalog No.: IHSIGAKT

$495

Category: Assay and ELISA Kits

  • Description

  • SDS

  • Sample Datasheet

  • Info Request

Product Description

Horse IgA ELISA Kit


Horse IgA ELISA Kits are highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IgA in biological samples of Horses. In this assay the IgA present in samples reacts with the anti-IgA antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgA antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgA. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3,5,5-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgA in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgA in the test sample. The quantity of IgA in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Contact Us About This Product

Horse IgA ELISA Kit

© 2018 Innovative Research. All Rights Reserved.