Dog Myoglobin ELISA Kit
Dog Myoglobin ELISA Kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring myoglobin in biological samples of Dogs.The myoglobin test kit is based on the principle of a solid phase enzyme-linked immunosorbent assay (ELISA). A monoclonal anti-myoglobin antibody is used for solid phase immobilization (on the microtiter wells) and a polyclonal anti- myoglobin antibody conjugated to horseradish peroxidase (HRP) is used for detection. The test sample is allowed to react simultaneously with the two antibodies, and the myoglobin molecules are thus sandwiched between the solid phase and enzyme-linked antibodies. After one hour incubation at room temperature, the wells are washed to remove unbound labeled antibodies. TMB (tetramethylbenzidine) Reagent is added and incubated for 20 minutes, resulting in the development of a blue color. The color development is stopped with the addition of Stop Solution, changing the color to yellow. The concentration of myoglobin in the test sample is proportional to the absorbance measured spectrophotometrically at 450 nm.