Cyno Monkey Factor VIII Total Antigen ELISA Kit
This cynomolgus macaque (Macaca fascicularis) monkey coagulation Factor VIII antigen assay is intended for the quantitative determination of total Factor VIII antigen in cyno plasma. For research use only.
Cyno Factor VIII will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled anti-cyno Factor VIII primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of cyno plasma. Color development is proportional to the concentration of Factor VIII in the samples.