Chicken Alpha-1 Acid Glycoprotein ELISA Kit
The Chicken Alpha-1 Acid Glycoprotein ELISA Kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring AGP in biological samples of chickens. In this assay, the AGP present in samples reacts with the anti-AGP antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-AGP antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound AGP. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3,5,5-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of AGP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of AGP in the test sample. The quantity of AGP in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.