Canine PAI-1 Total Antigen ELISA Kit
Plasminogen activator inhibitor-1 (PAI-1) is a central regulator of the blood fibrinolytic system. Clinical studies have indicated that increased PAI-1 levels increase the risk for thrombosis, whereas decreased levels may cause recurrent bleeding.
Dog PAI-1 total assay is intended for the quantitative determination of total plasminogen activator inhibitor type 1 (PAI-1) in dog plasma. The assay measures PAI-1 antigen in the 0.2 - 100 ng/ml range. If the unknown is thought to have high PAI 1 levels, dilutions may be made in blocking buffer. A 1:10-1:100 dilution for normal dog plasma is suggested for best results. Strip well format. Reagents for up to 96 tests.
Dog PAI-1 present in plasma reacts with the capture antibody coated and dried on a microtiter plate. Free, latent, and complexed PAI-1 will bind to the plate. After appropriate washing steps, primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with horseradish peroxidase conjugated streptavidin. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of dog PAI-1. The amount of color development is directly proportional to the concentration of total PAI-1 in the sample.
Detection Target: PAI-1
Reactivity: Dog / Canine
Range: 0.2 - 100 ng/ml
Sensitivity: 0.071 ng/ml
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