Bovine Plasminogen ELISA Kit (Urine and Cell Culture Samples)The Bovine Plasminogen ELISA kit is designed for detection of bovine plasminogen in urine and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures bovine plasminogen in less than 4 hours. A polyclonal antibody specific for bovine plasminogen has been pre-coated onto a 96-well microplate with removable strips. Plasminogen in standards and samples is sandwiched by the immobilized antibody and the biotinylated polyclonal antibody specific for bovine plasminogen, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.