Armenian Hamster IgG Antigen ELISA Kit
IgG is the most abundant immunoglobulin in serum and is predominately involved in the secondary immune response. The IgG subclasses are designated 1, 2, 3 and 4 based on their relative prevalence in human serum.
This Armenian hamster Immunoglobulin G (IgG) antigen assay is intended for the quantitative determination of total Armenian hamster IgG antigen in serum, plasma, hybridoma cell supernatants, ascites or other biological fluids. The assay measures total Armenian hamster IgG antigen in the 2 - 1,000 ng/ml range. If the unknown is thought to have high IgG levels, dilutions may be made in blocking buffer. A 1:100,000 to 1:1,000,000 dilution for normal Armenian hamster serum or plasma is suggested for best results.Strip well format. Reagents for up to 96 tests.
Armenian hamster IgG will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, horseradish peroxidase labeled polyclonal anti-Armenian hamster IgG primary antibody binds to the captured protein. Excess antibody is washed away and TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of Armenian hamster IgG. Color development is directly proportional to the concentration of total IgG in the samples.
Detection Target: IgG
Reactivity: Armenian Hamster
Range: 2 - 1000 ng/ml
Sensitivity: 1.08 ng/ml
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