Rat tPA Assay and ELISA Kits


Catalog No.: IRTPAKT


Category: Assay and ELISA Kits

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Product Description


tPA is a serine protease that converts plasminogen to the active serine protease plasmin in the blood fibrinolytic system. It also plays an important role in the removal of incipient thrombi. tPA is widely used for the thrombolytic treatment of acute myocardial infarction.

This rat tPA activity assay is intended for the quantitative determination of active tissue plasminogen activator (tPA) in rat plasma and other biological fluids. The assay measures active tPA in the 0.05 - 50 ng/ml range. If the unknown is thought to have high tPA levels, dilutions may be made in blocking buffer. Plasma and serum samples should be applied directly to the plate without dilution. Strip well format. Reagents for up to 96 tests.

Functionally active rat tPA will form a covalent complex with the biotinylated human PAI-1 which is bound to the avidin on the plate. Only free active tPA will react with the PAI-1 bound to the plate. After appropriate washing steps, polyclonal anti-tPA primary antibody binds to the captured tPA. Excess antibody is washed away and bound primary antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. Following an additional washing step, TMB substrate is used for color development at 450nm. The amount of color development is directly proportional to the concentration of active tPA in the sample.

Detection Target: tPA
Reactivity: Rat
Range: 0.05 - 50 ng/ml
Sensitivity: 0.01 ng/ml

Disclaimer: This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herein are offered for informational purposes only to be used solely for your consideration, investigation, and verification.

Antithrombotic Effects of Pyridinium Compounds Formed from Trigonelline upon Coffee Roasting

B Kalaska, L Piotrowski, A Leszczynska, B Michalowski - Journal of Agricultural and , 2014
Rat Tissue Plasminogen Activator (t-PA) activity kit was obtained from Innovative Research Inc. (Novi, MI, USA). Reagents to prothrombin time (PT), activated partial thromboplastin...


Noble-Collip drum trauma induces disseminated intravascular coagulation but not acute coagulopathy of trauma-shock

M Hayakawa, S Gando, Y Ono, T Wada, Y Yanagida - Shock, 2014
The antigen level of functionally active t-PA, which is not inactivated by plasminogen activator inhibitor, was determined using the Rat tPA Active ELISA Kit (IRTPAKT, Innovative Research, Inc., Novi, MI, USA). The antigen level of activated protein C was determined using...

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