Porcine tPA ELISA Kit
tPA is a serine protease that catalyzes the activation of plasminogen to plasmin. Clinical studies have indicated that high tPA levels may increase the risk for thrombosis, whereas decreased levels may cause neuronal plasticity and degeneration.
This porcine tissue-type plasminogen activator (tPA) activity assay is intended for the quantitative determination of active tPA in porcine plasma and other biological fluids. This assay measures active tPA in the 0.02 - 10 ng/ml range. If the unknown is thought to have high tPA levels, dilutions may be made in blocking buffer. Strip well format. Reagents for up to 96 tests.
Functionally active tPA will form a covalent complex with biotinylated human PAI-1 which is bound to the avidin coated on the microtiter plate. Complexed tPA will not bind to the PAI-1 and will not be detected by the assay. After appropriate washing steps, polyclonal anti-porcine tPA primary antibody binds to the captured tPA. Excess antibody is washed away and bound polyclonal antibody is reacted with the secondary antibody conjugated to horseradish peroxidase. Following an additional washing step, TMB is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of porcine tPA. The amount of color development is directly proportional to the concentration of active tPA in the sample.
Detection Target: TPA
Range: 0.02 - 10 ng/ml
Sensitivity: 0.018 ng/ml
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