Mouse tPA Assay and ELISA Kits

Mouse tPA ELISA Kit

Catalog No.: IMTPAKT


Category: Assay and ELISA Kits

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Product Description

Mouse tPA ELISA Kit

Mouse Tissue Plasminogen Activator (tPA) ELISA Kit is for the sensitive measurement of functionally active mouse tPA is easily performed with this Mouse tPA ELISA Kit. Active tPA will bind to biotinylated functionally active PAI-1 bound to the avidin coated microtiter plate. Only free active enzyme will react with the PAI-1 on the plate. Tissue plasminogen activator is a serine protease that converts plasminogen to plasmin in the blood fibrinolytic system. It also plays an important role in the nervous system, including the processes of neuronal migration, neurite outgrowth, and neuronal plasticity. tPA has been suggested to have a role in several neuropathological conditions such as cerebral ischemia, seizures, and demyelinating diseases.

The sensitive quantitative measurement of functionally active mouse tPA in plasma and other biological fluid samples is easily performed with this 96 well strip format ELISA kit. The level of active tPA in normal plasma was found to be 9.9 ng/ml in NSA/CF-1, 1.4 ng/ml in C57BL6, and 0.4 ng/ml in CD-1 mouse strains. The assay measures active tPA in the 0.05-50 ng/ml range. Samples giving mouse tPA levels above 50 ng/ml should be diluted in blocking buffer before use. Samples of plasma in citrate or EDTA may be assayed with this kit. Plasma in heparin is not recommended. It is important to ensure a platelet free preparation of plasma as platelets can release PAI-1, which in turn could potentially form a complex with active tPA. If plasma samples were collected in citrate the pH should be brought up to neutral with the 10X TBS provided in the kit. Serum and cell culture media at neutral pH may also be used. Functionally active tPA will form a covalent complex with the biotinylated human PAI-1 which is bound to the avidin on the plate.

Complexed tPA will not bind to the PAI-1 and will not be detected by the assay. After appropriate washing steps, anti-mouse tPA primary antibody binds to the captured enzyme. Excess antibody is washed away, and bound antibody is reacted with the secondary antibody conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of active tPA in the samples. A standard calibration curve is prepared using dilutions of purified tPA and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

Endothelial Progenitor Populations in Differentiating Embryonic Stem Cells. II. Drug Selection and Functional Characterization

S Kim, HA von Recum - Tissue Engineering Part A, 2009 - online.liebertpub.com
...section. Both Mouse tPA Activity Assay (cat. no. IMTPAKT; Innovative Research, Novi, MI) and Mouse tPA Total Antigen Assay (cat. no. IMTPAKT-TOT; Innovative Research) were used according to the manufacturer's protocol. tPA...


Fibrinolysis is down-regulated in mouse collagen-induced arthritis, but its normalization does not alleviate the course of disease

J Kwiecinski, E Josefsson... - Inflammation Research, 2011 - Springer
...Plasma levels of PAI-1 were measured with Murine PAI-1 Total Antigen Assay (Innovative Research, Novi, MI, USA), plasma active tPA levels were measured with Mouse tPA Activity Elisa Assay (Innovative Research) and plasma levels of D-dimer were measured with...

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Mouse tPA ELISA Kit

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