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Mouse tPA Assay and ELISA Kits

Mouse tPA ELISA Kit

Catalog No.: IMTPAKT

$495

Category: Assay and ELISA Kits

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Product Description

Mouse tPA ELISA Kit


Tissue plasminogen activator (tPA) is a serine protease that converts plasminogen to plasmin in the blood fibrinolytic system. It also plays an important role in the nervous system, including the processes of neuronal migration, neurite outgrowth, and neuronal plasticity. tPA has been suggested to have a role in several neuropathological conditions such as cerebral ischemia, seizures, and demyelinating diseases.

This mouse tPA activity assay is intended for the quantitative determination of active tissue plasminogen activator in mouse plasma and other biological fluids. The assay measures active tPA antigen in the 0.05 - 50 ng/ml range. If the unknown is thought to have high tPA levels, dilutions may be made in blocking buffer. Plasma samples should be applied directly to the plate without dilution. Strip well format. Reagents for up to 96 tests.

Functionally active mouse tPA will bind to the biotinylated human PAI-1 coated on the microtiter plate. Only free active enzyme will react with the PAI-1 on the plate. A standard calibration curve is prepared using dilutions of tPA along with the samples to be measured. After appropriate washing steps, monoclonal anti-mouse tPA primary antibody binds to the captured enzyme. Excess antibody is washed away and bound monoclonal antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. Following an additional washing step, TMB is used for color development at 450nm. The amount of color development is directly proportional to the concentration of active tPA in the sample.

Detection Target: TPA
Reactivity: Mouse
Range: 0.05 - 50 ng/ml
Sensitivity: 0.014 ng/ml

Disclaimer: This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herein are offered for informational purposes only to be used solely for your consideration, investigation, and verification.
(12-30-09)

Endothelial Progenitor Populations in Differentiating Embryonic Stem Cells. II. Drug Selection and Functional Characterization


S Kim, HA von Recum - Tissue Engineering Part A, 2009 - online.liebertpub.com
...section. Both Mouse tPA Activity Assay (cat. no. IMTPAKT; Innovative Research, Novi, MI) and Mouse tPA Total Antigen Assay (cat. no. IMTPAKT-TOT; Innovative Research) were used according to the manufacturer's protocol. tPA...

(7-24-11)

Fibrinolysis is down-regulated in mouse collagen-induced arthritis, but its normalization does not alleviate the course of disease


J Kwiecinski, E Josefsson... - Inflammation Research, 2011 - Springer
...Plasma levels of PAI-1 were measured with Murine PAI-1 Total Antigen Assay (Innovative Research, Novi, MI, USA), plasma active tPA levels were measured with Mouse tPA Activity Elisa Assay (Innovative Research) and plasma levels of D-dimer were measured with...

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Mouse tPA ELISA Kit

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