Mouse PAI-1 Assay and ELISA Kits

Mouse PAI-1 ELISA Kit

Catalog No.: IMPAIKT


Category: Assay and ELISA Kits

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Product Description

Mouse PAI-1 ELISA Kit

Mouse PAI-1 ELISA Kit for the sensitive measurement of functionally active mouse PAI-1 is easily performed with this 96 well strip format ELISA kit. Functionally active PAI-1 present in plasma or culture media reacts with urokinase coated onto a micro titer plate. Latent or complexed PAI-1 will not bind to the plate and will not be detected. After appropriate washing steps, anti mouse PAI-1 primary antibody binds to the PAI-1.

Functionally active PAI-1 present in plasma reacts with urokinase coated and dried on a microtiter plate. Latent or complexed PAI-1 will not bind to the plate and will not be detected. Unbound PAI-1 samples are washed away and an anti-PAI-1 primary antibody is added. Excess primary antibody is washed away and bound antibody, which is proportional to the original active PAI-1 present in the samples, is then reacted with the horseradish peroxidase secondary antibody. Following an additional washing step, TMB is then used for color development at 450 nm. The amount of color development is directly proportional to the concentration of active PAI-1 in the sample.

Neutralization of the antifibrinolytic function of plasminogen activator inhibitor1 resolves skin fibrosis

R Lemaire, T Burwell, H Sun, T Delaney, J Bakken - Arthritis & Rheumatology, 2015
... Mouse active PAI-1 and active plasmin levels were determined in plasma and skin lysates using ELISA kits as instructed by manufacturer (Innovative Research (Novi, Michigan). RNA analysis. Murine skin tissues were homogenized using a Polytron (Kinematica, Bohemia, ...


The Efficacy and Tolerability of Azilsartan in Obese Insulin Resistant Mice with Left Ventricular Pressure Overload

AKMT Zaman, DL McLean, BE Sobel - ... of Cardiovascular Pharmacology (in this issue ..., 2013
...PAI-1 was measured in supernatant fractions of homogenates and in plasma samples by ELISA with kits supplied by Innovative Research, Inc., Novi, MI. Supernatant fractions were assayed also for total protein (44) and total or isozyme Page 8.


HDL3, but not HDL2, stimulates plasminogen activator inhibitor-1 release from adipocytes: the role of sphingosine-1-phosphate

MH Lee, SM Hammad, AJ Semler, LM Luttrell... - ... of lipid research, 2010 - ASBMB
...Quantitative analyses. PAI-1. The concentration of mouse PAI-1 protein secreted into the media by cultured adipocytes was determined using a commercially available ELISA assay (Innovative Research, Novi, MI). This assay...

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Mouse PAI-1 ELISA Kit

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