Human PAI-1 ELISA Kit
Human PAI-1 ELISA Kit is for the sensitive measurement of functionally active PAI-1 in human plasma samples. Functionally active PAI-1 present in plasma reacts with urokinase coated onto a micro titer plate. Latent or complexed PAI-1 will not bind to the plate and will not be detected. After appropriate washing steps, anti human PAI-1 primary antibody binds to the PAI-1. Excess antibody is washed away, and bound antibody, which is proportional to the original active PAI-1 present in the plasma sample, is then reacted with the secondary antibody conjugated to HRP. TMB substrate is used for color development at 450 nm.
Functionally active PAI-1 present in plasma reacts with urokinase coated and dried on a microtiter plate. Latent or complexed PAI-1 will not bind to the plate or be detected. Unbound PAI-1 samples are aspirated and an anti-PAI-1 primary antibody is added. Excess primary antibody is then aspirated. The bound antibody, which is proportional to the original active PAI-1 present in the samples, is then reacted with the horseradish peroxidase conjugated secondary antibody. Following an additional washing step, TMB substrate solution is then used for color development at 450nm. The amount of color development is directly proportional to the concentration of active PAI-1 in the sample.
A standard calibration curve is prepared using dilutions of purified PAI-1 and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.