Human PAI-1 ELISA Kit
PAI-1 is involved in the regulation of the blood fibrinolytic system. Increased plasma levels of PAI-1 are implicated in the impairment of fibrinolytic function and may be associated with thrombotic diseases. Levels of PAI-1 increase with age and are elevated in conditions such as normal pregnancy and sepsis.
This human PAI-1 activity assay is for the quantitative determination of active plasminogen activator inhibitor type 1 (PAI-1) in human plasma. The assay measures active PAI-1 in the 0.125 - 200 U/ml range. If the unknown is thought to have high PAI-1 levels, dilutions should be made in plasma depleted of PAI 1 (cat IHPLA-SC-PAI-tPA) or in additional zero unit standard. Strip well format. Reagents for up to 96 tests.
Functionally active PAI-1 present in plasma reacts with urokinase coated and dried on a microtiter plate. Latent or complexed PAI-1 will not bind to the plate or be detected. Unbound PAI-1 samples are aspirated and an anti-PAI-1 primary antibody is added. Excess primary antibody is then aspirated. The bound antibody, which is proportional to the original active PAI-1 present in the samples, is then reacted with the horseradish peroxidase conjugated secondary antibody. Following an additional washing step, TMB substrate solution is then used for color development at 450nm. The amount of color development is directly proportional to the concentration of active PAI-1 in the sample.
Detection Target: PAI-1
Range: 0.125 - 200 U/ml
Sensitivity: 0.11 U/ml
Disclaimer: This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herein are offered for informational purposes only to be used solely for your consideration, investigation, and verification.