Rat Factor XII Total Antigen ELISA KitThis rat coagulation Factor XII antigen assay is intended for the quantitative determination of total FactorXII antigen in rat plasma.
Rat Factor XII will bind to the affinity purified capture antibody coated on the microtiter plate. Factor XII and XIIa will react with the antibody on the plate. After appropriate washing steps, biotin labeled anti rat Factor XII primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of rat Factor XII. Color development is proportional to the concentration of Factor XII in the samples.