Rat Factor XII Total Antigen ELISA Kit
Factor XII (aka Hageman Factor) is a single-chain, 615 amino acid glycoprotein zymogen. Factor XII is activated by kallikrein. Factor XIIa converts prekallikrein to kallikrein during the intrinsic pathway of the coagulation cascade. Although Factor XII is not thought to play an essential role in normal hemostasis, lack of Factor XII in a mouse model resulted in a 'severe defect' in thrombus formation.
This rat coagulation Factor XII antigen assay is intended for the quantitative determination of total Factor XII antigen in rat plasma. The assay measures Factor XII antigen in the 0.02 - 10 ng/ml range. If the unknown is thought to have high Factor XII levels, dilutions may be made in blocking buffer. A 1:10,000-1:100,000 dilution for normal rat plasma is suggested for best results.Strip well format. Reagents for up to 96 tests.
Rat Factor XII will bind to the affinity purified capture antibody coated on the microtiter plate. Factor XII and XIIa will react with the antibody on the plate. After appropriate washing steps, biotin labeled anti rat Factor XII primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of rat Factor XII. Color development is proportional to the concentration of Factor XII in the samples.
Detection Target: Factor XII
Range: 0.02 - 10 ng/ml
Sensitivity: 0.011 ng/ml
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