Human TNFsR I ELISA Kit - 1 Kit (96 Wells)Our Human TNFsR I ELISA Kit is a sandwich ELISA intended for the quantitative determination of human TNF s RI in cell culture supernates, serum, plasma (heparin, EDTA, citrate) and urine.
TNFsRI(also known as Solube tumor necrosis factor receptor 1) is a cytokine receptor that binds tumor necrosis factors(TNF). In contrast, the p60(TNFRSF1A) and p80(TNFRSF1B) TNFA receptors self-assemble through a distinct functional domain in the TNFR extracellular domain, termed the pre-ligand assembly domain(PLAD), in the absence of ligand. Deletion of the PLAD results in monomeric presentation of p60 or p80. Flow cytometric analysis showed that efficient TNFA binding depends on receptor self-assembly. They also found that other members of the TNF receptor superfamily, including the extracellular domains of TRAIL(TNFRSF10A), CD40, and FAS(TNFRSF6), all self-associate but do not interact with heterologous receptors. By Southern blot analysis of human/Chinese hamster somatic cell hybrid DNA, the TNFR1 gene was mapped to 12pter-cen. And by nonradioactive in situ hybridization that the type 1 receptor(the p55 TNF receptor) is encoded by a gene located on chromosome 12p13.2.