Human Plasmin-Antiplasmin Complex KitHuman plasmin/antiplasmin (PAP) complex assay is intended for the quantitative determination of the covalent complex of plasmin and its inhibitor ?2-antiplasmin in human plasma, serum, urine, cell culture media, and tissue extracts.
Human PAP complex in samples will bind to the anti-human plasmin capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled polyclonal anti-2-antiplasmin primary antibody binds to PAP complex captured on the plate. Excess antibody is washed away and bound polyclonal antibody is then reacted with horseradish peroxidase conjugated streptavidin. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of PAP complex. Color development is proportional to the concentration of PAP complex in the samples. Free plasminogen and antiplasmin will not be detected by this assay.