Human Plasmin-Antiplasmin Complex Kit
Plasminogen is a single chain glycoprotein zymogen and is the precursor of the fibrinolytic enzyme plasmin. The serine protease plasmin rapidly forms a 1:1 covalent complex with its major circulating inhibitor Alpha2-antiplasmin. Detection of PAP complex in plasma represents activation of the fibrinolytic system either directly or secondarily to coagulation in vivo.
Human plasmin/antiplasmin (PAP) complex assay is intended for the quantitative determination of the covalent complex of plasmin and its inhibitor Alpha2-antiplasmin in human plasma, serum, urine, cell culture media, and tissue extracts. The assay measures PAP complex in the 1 - 500 ng/ml range. If the unknown is thought to have high PAP complex levels, dilutions may be made in blocking buffer. Plasma samples should be applied directly to the plate without dilution. Strip well format. Reagents for up to 96 tests.
Human PAP complex in samples will bind to the anti-human plasmin capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled polyclonal anti-alpha2-antiplasmin primary antibody binds to PAP complex captured on the plate. Excess antibody is washed away and bound polyclonal antibody is then reacted with horseradish peroxidase conjugated streptavidin. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of PAP complex. Color development is proportional to the concentration of PAP complex in the samples. Free plasminogen and antiplasmin will not be detected by this assay.
Detection Target: Plasmin Antiplasmin Complex (PAP)
Range: 1 - 500 ng/ml
Sensitivity: 0.094 ng/ml
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