Cyno Monkey Plasminogen Total Antigen ELISA Kit
Plasminogen is a single chain glycoprotein zymogen and is the precursor of the fibrinolytic enzyme plasmin. Plasminogen deficiencies are classified as hypoplasminogenemia (Type I) or dysplasminogenemia (Type 2) and are associated with decreased extracellular fibrin clearance leading to mucous membrane lesions and ligneous conjunctivitis.
This cynomolgus macaque (Macaca fascicularis) monkey plasminogen total assay is for the quantitative determination of total plasminogen antigen in cyno monkey plasma, serum, or cell culture media. The assay measures plasminogen and plasmin antigens in the 0.5 - 100 ng/ml range. If the unknown is thought to have high plasminogen/plasmin levels, dilutions may be made in blocking buffer. A 1:10,000-1:100,000 dilution for normal cyno plasma is suggested for best results. Strip well format. Reagents for up to 96 tests.
Cyno monkey plasminogen will bind to the affinity purified capture antibody coated on the microtiter plate. Plasminogen, plasmin, and plasmin in complex with antiplasmin will react with the antibody on the plate. After appropriate washing steps, biotin labeled polyclonal anti-cyno plasminogen primary antibody binds to the captured protein. Excess antibody is washed away and bound polyclonal antibody is then reacted with peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of cyno plasminogen. Color development is proportional to the concentration of total plasminogen in the sample.
Detection Target: Plasminogen
Reactivity: Cyno Monkey
Range: 0.5 - 100 ng/ml
Sensitivity: 0.243 ng/ml
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