Cyno Monkey IgG Antigen ELISA Kit
IgG is the most abundant immunoglobulin in serum and is predominately involved in the secondary immune response. The IgG subclasses are designated 1, 2, 3 and 4 based on their relative prevalence in human serum.
This cynomolgus macaque (Macaca fascicularis) monkey Immunoglobulin G (IgG) antigen assay is intended for the quantitative determination of total IgG antigen in cyno monkey serum, plasma, hybridoma cell supernatants, ascites or other biological fluids. The assay does not distinguish IgG subclasses. The assay measures total cyno IgG antigen in the 0.1 - 100 ng/ml range. If the unknown is thought to have IgG levels greater than 100ng/ml, dilutions may be made in blocking buffer before use. A 1:1,000,000-1:10,000,000 dilution for normal cyno monkey plasma is suggested for best results. Strip well format. Reagents for up to 96 tests.
Cyno monkey IgG will bind to the monoclonal capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled monoclonal anti-cyno IgG primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with horseradish peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of cyno monkey IgG. Color development is proportional to the concentration of IgG in the samples.
Detection Target: IgG
Reactivity: Cynomolgus Macaque (Macaca Fascicularis) Monkey
Range: 0.1 - 100 ng/ml
Sensitivity: 0.035 ng/ml
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