Non Swiss Albino Mouse Serum Reference

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Written by admin on December 4, 2013. Posted in Blog

Non Swiss Albino Mouse Serum 1Innovative Research's NSA Mouse Serum was recently used for the below research. The entire article is available at ScienceDirect.com.

A quantitative LC-MS/MS method for determination of thiazolidinedione mitoNEET ligand NL-1 in mouse serum suitable for pharmacokinetic studies

KK Pedada, X Zhou, H Jogiraju, RT Carroll... - Journal of Chromatography ..., 2013 ... Millipore (Billerica, MA, USA). Six different lots of Non Swiss Albino Mouse serum were from Innovative Research (NoviMI, USA). Dosed mouse serum samples were obtained at the indicated time points. All animal procedures ...

Abstract:

(Courtesy of ScienceDirect.com) Thiazolidinedione (TZD) compounds have shown promise as antidiabetic, antibiotics, antifungal and neuroprotective agents. The mitochondrial effect of a novel mitoNEET ligand, NL-1 {5-[(3,5-di-tert-butyl-4-hydroxyphenyl)methyl]-1,3-thiazolidine-2,4-dione}, and other TZD compounds, is a newly proposed mechanism for the neuroprotective action of these TZD compounds. In this work, a sensitive LC-MS/MS assay has been developed and validated for quantification of NL-1 in mouse serum. Sample preparation involved an acetonitrile protein precipitation procedure with addition of an internal standard NL-2 {5-[(4-hydroxy-3,5-dimethyl-phenyl)methyl]thiazolidine-2,4-dione}. LC-MS/MS analysis utilized a Columbus C-18 HPLC column (2 × 50 mm, 5 μm). Chromatography employed a multiple step gradient program that featured a steep linear gradient (25% to 95% in 0.5 min) of 15 μM ammonium acetate (additive for eliminating carry-over) in 2% methanol mixing with increasing proportions of 100% methanol. The HPLC was interfaced to a QTrap 5500 mass spectrometer (AB Sciex) equipped with an electrospray ionization source used in a negative ionization mode. Multiple reaction monitoring (MRM) of m/z 334 → 263 for NL-1 and m/z 250 → 179for NL-2 was done. The method had a linear range of at least 1-100 ng/mL in serum. The intra-assay and inter-assay coefficient of variation (%CV) were less than 4% and accuracies (%RE) ranged from -2.7% to 2.0%. The analytical procedure gave 96 -115% absolute extraction recovery of NL-1. The relative matrix effect was measured and found to be insignificant. The analyte in serum was confirmed to be stable during storage and treatment. The method is suitable for pharmacokinetic (PK) studies of the parent drug NL-1 based on the preliminary serum results from dosed NL-1 mouse studies

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