New References Published
For urine experiments, normal human urine was purchased from Innovative Research (Novi, MI) and stored at 4 C until used. Other chemicals were analytical reagent grade, and used as received. All samples
Engineering Polypeptide Coatings to Augment Gene Transduction and in vivo; Stability of AdenovirusesZK Jiang, SBS Koh, M Sato, IC Atanasov, M Johnson... - Journal of Controlled ..., 2012
Human serum (pooled from 10 donors) was purchased from Innovative Research, Inc., Novi, MI. Ad or Ad-K60KP370 was applied to AML-12 cells in serum-free media or serum-free media containing blood clotting factor, Factor X (FX), at physiological concentration (8 g/ml).
Twenty μl of culture supernatant was added to 100 μl phosphate buffered saline (PBS), 10 μl human plasma (Innovative Research, Novi, MI), and10 μl S2403 (1 mg/ml) (Diapharma group Inc., West Chester, OH) and incubated at 37uC for 2 hours.