Human Serum References

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Written by admin on March 25, 2015. Posted in Blog

Innovative Research's Pooled Normal Human Serum was recently used in the following studies:

Immunoproteomically Identified GBAA_0345, Alkyl Hydroperoxide Reductase Subunit C is a Potential Target for Multivalent Anthrax Vaccine

YH Kim, K Kim, YR Kim, MK Choi, HK Kim, KJ Choi... - Proteomics, 2013
immunoproteomic analyses. A pooled sample of normal human sera was purchased from Innovative Research Inc. (Peary Court Novi, MI, USA). Sera from five patients with anthrax werealso used in this study. These patients contracted anthrax during Korean outbreaks in



Outer membrane protein P5 is required for resistance of nontypeable Haemophilus influenzae to both the classical and alternative complement pathways.

CV Rosadini, S Ram, BJ Akerley - Infection and Immunity, 2013
and incubated at 37C for 30min with or without pooled normal human serum (NHS) from 158healthy anonymous donors age 18-65 (final concentrations specified in the figures) (Innovative Research, Novi, Michigan)

Application of 31P NMR spectroscopy and chemical derivatization for metabolite profiling of lipophilic compounds in human serum

MA DeSilva, N Shanaiah... - Magnetic ..., 2009 - Wiley Online Library
and used without further purification. Deuterated solvents were purchased from Cambridge isotope (Andover, MA). Human serum samples were obtained from Innovative Research (Novi, MI).



Staphylococcus aureus</i> resistance on titanium coated with multivalent PEGylated-peptides

X Khoo, GA O'Toole, SA Nair, BD Snyder... - Biomaterials, 2010 Elsevier
saturated peptide coating. Beads were washed three times in TBS-T then transferred into 100% human serum (Innovative Research; Novi, MI) and kept at room temperature on an orbital shaker. After various serum exposure



The TonB3 system in the human pathogen Vibrio vulnificus is under the control of the global regulators Lrp and CRP.

AF Alice... - Journal of Bacteriology, 2012 - Am Soc Microbiol
at -80C. In some experiments commercial pooled normal human serum was used (Cat 106 IPLA-SER, Innovative Research, Novi, MI) with similar results to those obtained from 107 other source. Ferric ammonium citrate (200 μg/ml) (FAC) was added to allow V. 108



The use of strong anionexchange (SAX) magnetic particles for the extraction of therapeutic siRNA and their analysis by liquid chromatography/mass spectrometry

G Ye... - Rapid Communications in Mass ..., 2011 - Wiley Online Library
...Human serum used for the siRNA stability studies was obtained from Innovative Research (Novi, MI, USA). Strong anion-exchange (SAX) beads were purchased from Invitrogen (Dynabeads SAX, Cat No. 105.15D; Carlsbad, CA, USA) and Bioclone Inc.



Quantification of monosaccharides through multiplereaction monitoring liquid chromatography/mass spectrometry using an aminopropyl column LA Hammad, DZ Derryberry... - ... in Mass Spectrometry, 2010 - Wiley Online Library
Louis, MO, USA). PNGase F (5000 units/mL) was obtained from Northstar BioProducts (East Falmouth, MA, USA), and human blood sera were obtained from Innovative Research (Novi, Michigan, USA). C18 and active charcoal...



Development of a Sensitive Bead-Based Assay for Enhanced Monoclonal Antibody Detection

ME Ruidaz, N Mendez, AB Sanchez... - MRS ..., 2011 - Cambridge Univ Press
of 100ul of 3% BSA solution, 10ul of PBS with a specific concentration of monoclonal alemtuzumab antibody as obtained from the UCSD cancer center pharmacy (Genzyme, Cambridge, MA), 10ul of human serum, purchased from Innovative Research (Novi, MI) and Valley



New neutralizing antibody epitopes in hepatitis C virus envelope glycoproteins are revealed by dissecting peptide recognition profiles

A Kachko, G Kochneva, G Sivolobova... - Vaccine, 2011 - Elsevier
counted as positive. Negative controls were represented by normal mouse serum (NMS); normal human serum (Innovative Research, Novi, MI) and chimpanzee sera obtained prior to immunization/challenge. Titres are expressed



ApoB-100-containing Lipoproteins Are Major Carriers of 3-Iodothyronamine in Circulation E Placzek, TS Scanlan - Journal of Biological Chemistry, 2012 - ASBMB
500 μl of normal pooled serum, human and rat serum from Innovative Research, Novi, MI; mouse serum from Millipore, Billerica, MA) was incubated with tracer quantities of 125 IT 1 AM for 24h at 4 C in the presence or absence of excess unlabeled T 1 AM (50 μm).



Modulation of Thyroid Hormone Concentrations in Serum of Rats Coadministered with Perchlorate and Iodide-Deficient Diet

T Kunisue, JW Fisher, K Kannan - Archives of environmental ..., 2011 - Springer
The accuracy and precision of the method were evaluated by the use of human serum (No. IR08-040) and rat serum (No. 71164736) purchased from Innovative Research (Novi, MI). A procedural recovery test was conducted by using human serum as a representative matrix.



The effect of physiological concentrations of sex hormones, insulin, and glucagon on growth of breast and prostate cells supplemented with unmodified human serum

A Esfahani, CWC Kendall, B Bashyam... - In Vitro Cellular & ..., 2010 - Springer
These were then diluted to the desired concentrations with serum-free medium. For the experiments, the media were supple- mented with 10% commercial human serum (pooled serum from male and females, Innovative Research Novi, MI).



Rapid, direct analysis of cholesterol by charge labeling in reactive desorption electrospray ionization

C Wu, DR Ifa, NE Manicke, RG Cooks - Analytical chemistry, 2009 - ACS Publications
(Portsmouth, NH). The serum sample, pooled from 10 individuals, was purchased from Innovative Research Inc.(Novi, MI) and stored in -20 C until analysis. The rat brains were purchased from Pel-Freez Inc. (Rogers, AR).



Selective enrichment and sensitive detection of peptide and protein biomarkers in human serum using polymeric reverse micelles and MALDI-MS

N Rodthongkum, R Ramireddy, S Thayumanavan... - Analyst, 2012 - xlink.rsc.org
biomarkers. Experimental section. Reagents. Pooled normal human serum was Acquired from Innovative Research (Novi, MI). ProteoSeek HSA/IgG removal kit was obtained from Pierce Biotechnology (Rockford, IL).



An efficient rHSV-based complementation system for the production of multiple rAAV vector serotypes

W Kang, L Wang, H Harrell, J Liu, DL Thomas... - Gene therapy, 2008 - nature.com
H 2 SO 4 and detected by reading absorbance 450 nm. Pooled human serum (Innovative Research, Novi, MI, USA) was used as the hAAT positive control quantified as l.5 mg ml -1 of -1-antitrypsin.



Encapsulation and Release of Amphotericin B from an ABC Triblock Fluorous Copolymer

JP Jee, A McCoy, S Mecozzi - Pharmaceuticalresearch, 2011 - Springer
1H,1H,12H,12H-Perfluoro-1,12-dodec- anediol was purchased from SynQuest Laboratories Inc. (Alachua, FL). Human serum was obtained from Innovative Research (Novi, MI USA). All solvents were of ACS grade or higher and were purchased from Sigma-Aldrich Inc.



"Add to Subtract": A Simple Method to Remove Complex Background Signals from the 1 H Nuclear Magnetic Resonance Spectra of Mixtures

D Raftery, T Ye, C Zheng, S Zhang, NA Gowda... - Analytical ..., 2012 - ACS Publications
subject with diabetes. Sample set 2: A human serum sample was purchased from Innovative Research (Novi, MI) and aliquoted. One aliquot was used without further processing for CPMG experiments. For comparison, proteins



Serum and plasma brain-derived neurotrophic factor (BDNF) in abstinent alcoholics and social drinkers

C D'Sa, RJ Dileone, GM Anderson, R Sinha - Alcohol, 2012 Elsevier
Low" and "High" concentration quality control (QC) pools were prepared by adding 10 ng or 100 ng to 5 ml portions of human serum (Innovative Research, Novi, MI, USA), giving nominal concentrations of 2 and 20 ng/ml, respectively....



Engineering Polypeptide Coatings to Augment Gene Transduction and< i> in vivo</i> Stability of Adenoviruses

ZK Jiang, SBS Koh, M Sato, IC Atanasov, M Johnson... - Journal of Controlled ..., 2012
Human serum (pooled from 10 donors) was purchased from Innovative Research, Inc., Novi, MI . Ad or Ad-K60KP370 was applied to AML-12 cells in serum-free media or serum-free media containing blood clotting factor, Factor X (FX), at physiological concentration (8 g/ml).



Proteolysis Breaks Tolerance Toward Intact 345 (IV) Collagen, Eliciting Novel Anti-Glomerular Basement Membrane Autoantibodies Specific for 345NC1 Hexamers

F Olaru, XP Wang, W Luo, L Ge, JH Miner, S Kleinau... - The Journal of Immunology, 2013
Normal human sera (Innovative Research, Novi, MI) were used as negative controls. Immunoassays ELISA was performed in MaxiSorp plastic plates coated overnight with NC1 monomers (100 ng/well) or NC1 hexamers (300 ng/well) and then blocked with 1% BSA.



RNA aptasensor for rapid detection of natively folded type A botulinum neurotoxin

P Janardhanan, CM Mello, BR Singh, J Lou, JD Marks... - Talanta, 2013
Molecular biology grade dithiothreitol (DTT) was obtained from Promega (Madison, WI). Pooled normal human serum was purchased from Innovative Research (Novi, MI). 2.2. Biotinylated RNA aptamer synthesis.





Innovative Research's Single Donor Human Serum was recently used in the following studies:

Development and validation of an LC-MS/MS method for determination of phencyclidine in human serum and its application to human drug abuse cases

KC Chimalakonda, C Hailey, R Black, A Beekman... - Anal. Methods, 2010 - xlink.rsc.org
Human O serum was obtained from Innovative Research, Inc., Peary Court Novi, Mich, USA Drug free human serum from individual donors was purchased from Innovative Research (Novi, MI). Drug free human whole blood was obtained from the Clinical Laboratory at the University of Arkansas Hospital (Little Rock, AR).



Inhibition of the Growth of Plasmodium falciparum in Culture by Stearylamine-Phosphatidylcholine Liposomes

GM Hasan, N Garg, E Dogra, R Surolia... - ... Parasitology Research, 2011 - hindawi.com
Human O serum was obtained from Innovative Research, Inc., Peary Court Novi, Mich, USA Mayer, LCL Tai, DSC Ko et al., "Influence of vesicle size, lipid composition, and drug-to-lipid ratio on the biological activity of liposomal doxorubicin in mice," Cancer Research





Innovative Research's Charcoal Stripped Pooled Human Serum was recently used in the following study:

An Improved Method for the Study of Apoptosis-related Genes using the tet-on System

MW Halterman - Journal of Biomolecular Screening, 2011 - jbx.sagepub.com
standard protocols maintained in DMEM/8% serum containing 200 g/mL hygromycin for maintenance. Stripped serum was used throughout to minimize basal pBig2i activity Innovative Research, Novi, MI. In vitro hypoxia model.

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