Do You Have An ELISA Kit For The Detection Of Serum Amyloid A (SAA) In The Rat?
We have been asked on many occasions if our SAA ELISA kit is cross-reactive with rat.
Published reports indicate that rat does indeed possess the gene for SAA, but that the gene contains a deletion. This gene appears to be transcribed and interestingly is under the control of a promoter, an inhibitory regulatory element, and a cytokine response element. Truncated mRNA for SAA can be detected, but the mature protein does not appear in serum or plasma samples.
Yamada, T., N. Hirano, T. Kuoda, Y. Okuda, and Y. Ito (1997) Generation and characterization of rat monoclonal antibodies against human serum amyloid A. Scand. J. Immunol. 46(2):175-179.
Marhaug, G. and S.B. Dowton (1994) Serum amyloid A: An acute phase apolipoprotein and precursor of AA amyloid. Baillieres. Clin. Rheumatol. 8(3):553-573.
Li, L. and W.S. Liao (1999) An upstream repressor element that contributes to hepatocyte-specific expression of the rat serum amyloid A1 gene. Biochem. Biophys. Res. Commun. 264:395-403.