Changes in spontaneous thrombolytic activity during progression of atherosclerosis in Apo-/- and LDLR-/- double knockout miceTakumi Sato, Manami Yoshimura, Tomohide Sanda, Kanae Hyodo, Junichiro Yamamoto, Hiromitu Ishii, Tsutomu Yamashita
International Journal of Clinical and Experimental Pathology
"... After 14 and 22 weeks of feeding the high-fat diet, blood was drawn from the abdominal aorta of mice into 3.14% sodium citrate by using a 23-gauge needle. All samples (citrated plasma) were stored at -80C until analysis. Plasma PAI-1 levels were determined by using a commercial ELISA kit (IMPAIKT-TOT, Innovative Research, Novi, Michigan, USA)..."
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