Human IgD ELISA Kit

The Human IgD ELISA Kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IgD in Human Biological products. Strip well format. Reagents for up to 96 tests. In this assay the IgD present in samples reacts with the anti-IgD antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-IgD antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgD. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3Æ,5,5Æ-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgD in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgD in the test sample. The quantity of IgD in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution. More Details: Detection Target: IgD Reactivity: Human Range: 6.25 ng/ml - 400 ng/ml Sensitivity: 3.197 ng/ml At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results. Please note ¢ this product replaces our old product number IRKTAH1134 (same product, new number).