IHP 1002- Human Prothrombin (Factor II)

Prepared from fresh frozen human plasma. Human Prothrombin is a glycoprotein of molecular weight 72,000, and consists of a single polypeptide chain. Activation of Prothrombin by Factor Xa yields the serine protease Thrombin. Prothrombin is homogeneous as judged by 10% SDS-PAGE gels and shows no reduction upon incubation with 2-mercaptoethanol. Buffer composition = 20 mM Tris-HCl/0.1 M NaCl /1 mM Benzamidine/pH 7.4 *Extinction Coefficient (1%) = 13.6 One unit = 90 µg Molecular Weight =72,000 daltons HT 1002a

Price:

100 units (9mg) $195
1,000 units (90 mg) $895

IH 1002a- Human a-Thrombin (Factor IIa)

Prepared from homogeneous human prothrombin by activation with Factor Xa, Factor Va, and phospholipid. Human Thrombin is homogeneous as judged by 10% SDS-PAGE gels. This activated enzyme has a minimum activity of 2,700 NIH units/mg when compared to NIH standard thrombin. Buffer composition = 50 mM Sodium Citrate/0.2 M NaCl/0.1% PEG-8000/pH 6.5 *Extinction Coefficient (1%) = 18.3 Molecular Weight = 37,000 daltons

Price:

<3,000 units- $95 per 1000 units
>3,000 units- $75 per 100 units

IHGT- Gamma Thrombin

A non-clotting derivative thrombin produced from homogeneous Human Thrombin by controlled incubation with immobilized Trypsin. Gamma-thrombin is a noncoagulant form of thrombin that retains much of its platelet-activating capacity. Gamma-thrombin is homogeneous as judged by 10% SDS-PAGE gels and shows complete reduction upon incubation with 2-mercaptoethanol. Buffer Composition = 10 mM Sodium Acetate/0.75 M NaCl/pH 6.0 Molecular Weight = 12,000 daltons Concentration determined by BCA

Price:

1.0 mg $495

IHFV- Human Factor V

Prepared from fresh frozen human plasma. Human Factor V is a single chain glycoprotein with a MW of 330,000. Human Factor V is activated by thrombin and less effectively by Factor Xa to yield the active cofactor Va. Human Factor Va, Factor Xa, and phospholipid form the prothrombinase complex which is responsible for the rapid activation of Prothrombin to Thrombin. Human Factor V is homogeneous as judged by a 4-20% gradient gel and shows no reduction upon incubation with 2-mercaptoethanol. Buffer =10 mM Tris-HCl/10 mM Boric Acid/1 mM CaCl2 /50% Glycerol/pH 7.2 *Extinction Coefficient (1%) = 9.6 Molecular Weight = 330,000 daltons

Price:

0.1 mg $595
0.5 mg $2,695

IHFVII 1007- Human Factor VII (Proconvertin)

Prepared from fresh frozen human plasma. Human Factor VII is a single-chain vitamin K dependent glycoprotein found in trace quantities in plasma (4 mg/Liter). It is a single band on 10% SDS-PAGE gels and shows no reduction upon incubation with 2-mercaptoethanol. In the Tissue Factor Pathway of coagulation, Human Factor Vlla, in the presence of calcium ions and tissue factor, activates Factors IX and X to their enzymatically active forms, Factor IXa and Xa. Buffer composition =20 mM Tris-HCl/0.1 M NaCl/1 mM Benzamidine/pH 7.4 *Extinction Coefficient (1%) = 13.9 Molecular Weight = 50,000 daltons

Price:

0.1 mg $295
1.0 mg $1,295

IHFVIIa- Human Factor VIIa

Prepared from purified Human Factor VII using Human Factor XIIa. The Factor Xlla is removed using our CTI column. Complete activation is observed on a 10% SDS-PAGE gel. The clotting activity is greater than 30,000 u/mg. This protein is a single band at 50,000 MW on a non-reduced gel and reduces to 29,500 and 23,500 with the addition of 2-mercaptoethanol. Factor Vlla, in the presence of calcium ions and Tissue factor, activates Factors IX and X to their enzymatically active forms, Factor IXa and Xa. Buffer composition = 20 mM Tris-HCl/0.1 M NaCl/pH 7.4 *Extinction Coefficient (1%) = 13.9 Molecular Weight = 50,000 daltons

Price:

0.1 mg $345
1.0 mg $1,595

IHPC-1001- Human Protein C

Purified from fresh frozen human plasma using a combination of salt precipitations and column chromatography, this protein is a single band on non-reducing 10% SDS-PAGE gels and shows total reduction upon incubation with 2-mercaptoethanol. No other coagulation factors are detected by functional assay. Protein C is activated to the serine protease, Activated Protein C (APC), by a-thrombin or the complex of a-thrombin/thrombomodulin and is a potent anticoagulant through the selective inactivation of Factors Va and VIIIa. Buffer composition = 20 mM Tris-HCl/0.1 M NaCl/1 mM Benzamidine/pH 7.4 *Extinction Coefficient (1%) = 14.5 Molecular Weight = 62,000 daltons

Price:

0.1 mg $295
1.0 mg $1,295

IAPC- Human Activated Protein C

Activated Protein C (APC) is a serine protease derived from the two chain vitamin K dependent zymogen, Protein C. APC inhibits blood coagulation through the selective inactivation of the cofactors Va and VIIIa. APC is prepared from homogeneous protein C by activation with purified thrombin. This thrombin is removed after activation by ion exchange chromatography. Activated Protein C is homogeneous on 10% SDS-PAGE gels and shows complete reduction upon incubation with 2-mercaptoethanol. Buffer composition = 20 mM Tris-HCl/0.1 M NaCl/ pH 7.4 *Extinction Coefficient (1%) = 14.5 Molecular Weight = 56,000 daltons

Price:

0.1 mg $295
1.0 mg $1,295

IHPS- Human Protein S

Protein S exists in two forms in human plasma, as the free protein and in complex with C4b-binding protein. Human protein S is a single-chain glycoprotein with a molecular weight of 69,000. HPS is homogeneous as judged on non-reducing SDS-PAGE gels and is a doublet upon incubation with 2-mercaptoethanol. Buffer composition = 20 mM Tris-HCl/0.1 M NaCl /1 mM Benzamidine/pH 7.4 *Extinction Coefficient (1%) = 9.5 One unit = 10 µg Molecular Weight = 69,000 daltons

Price:

1.0 mg $595

IHFIX1009- Human Factor IX (Christmas Factor)

Prepared from fresh frozen human plasma. This protein is homogeneous on 10% SDS-PAGE gels and shows no reduction upon incubation with 2-mercaptoethanol. No other coagulation factors are detected by functional assay. Using FIX deficient plasma as a substrate, Human Factor IX has an activity of approximately 175 units/mg of protein. Human Factor IX, activated by either the Contact or Tissue Factor Pathway, is responsible for the activation of Factor X to Xa. Buffer composition: 20 mM Tris-HCl/0.1 M NaCl /1 mM Benzamidine/pH 7.4 *Extinction Coefficient (1%) = 13.2 One unit = 5 µg Molecular Weight = 56,000 daltons

Price:

100 units (0.5mg) $295
500 units (2.5 mg) $1,295

IHFIXa 1070- Human Factor IXaa

Prepared from homogeneous Human Factor IX by activation with Russells' Viper Venom. This RVV-X cleaves a single internal Arg-Val peptide bond in the Factor IX. The RVV-X is removed after activation. Complete activation is observed on 10% SDS-PAGE gels. This protein is homogeneous on 10% SDS-PAGE gels and shows total reduction upon incubation with 2-mercaptoethanol. This form of activated Factor IX has about 50% of the coagulant activity of Human Factor IXab. Buffer composition = 20 mM Tris-HCl/0.1 M NaCl /pH 7.4 *Extinction Coefficient (1%) =14.9 One unit = 5 µg Molecular Weight = 56,000 daltons

Price:

100 units (0.5mg) $395
500 units (2.5 mg) $1,395

IHFIXa 1080- Human Factor IXab

Prepared from homogeneous Human Factor IX by activation with Bovine Factor XIa. This Bovine Factor XIa is removed after activation. Complete activation is observed on 10% SDS-PAGE gels. The Factor XIa activates Factor IX in a two-step reaction. In the first step, an internal Arg-Ala bond is cleaved, and in the second step, an Arg-Val bond is cleaved. The second cleavage leads to the liberation of an activation peptide from the NH2-terminal portion of the heavy chain to produce Factor IXab. Buffer composition = 20 mM Tris-HCl/0.1 M NaCl /pH 7.4 *Extinction Coefficient (1%) = 14.3 One unit =5 µg Molecular Weight = 45,000 daltons

Price:

100 units (0.5mg) $395
500 units (2.5 mg) $1,395

IHFX 1010- Human Factor X

Prepared from fresh frozen human plasma. This Vitamin K dependent protein is a single band on non-reducing 10% SDS-PAGE gels and shows total reduction upon incubation with 2-mercaptoethanol. No other coagulation factors are detected by functional assay. Using FX deficient plasma as a substrate, Human Factor X has an activity of approximately 100 units/mg of protein. Human Factor X, once activated via the Contact Factor Pathway or the Tissue Factor Pathway, is responsible for the conversion of Prothrombin to Thrombin. Buffer composition 20 mM Tris-HCl/0.1 M NaCl /1 mM Benzamidine/pH 7.4 *Extinction Coefficient (1%) =11.6 One unit =8 µg Molecular Weight =58,800 daltons

Price:

100 units (0.8mg) $295
500 units (4.0 mg) $1,095

IHFXA 1011- Human Factor Xa

Human Factor Xa is prepared from homogeneous Human Factor X by activation with Russels' Viper Venom. This RVV-X is removed after activation. Complete activation is observed on 10% SDS-PAGE gels. Factor Xa, as part of the prothrombinase complex along with the cofactor Va, phospholipids and Calcium ions, catalyzes the rapid conversion of prothrombin to thrombin. Buffer composition = 20 mM Tris-HCl/0.7 M NaCl/pH 7.4 *Extinction Coefficient (1%) =11.6 One unit = 8 µg Molecular Weight = 46,000 daltons

Price:

100 units (0.8mg) $395
500 units (4.0 mg) $1,195

IHFXI - Human Factor XI

Prepared from fresh frozen human plasma. Human Factor XI is a glycoprotein of molecular weight 160,000. This protein migrates as a single band on 10% SDS-PAGE gels in the absence of reducing agents. Human Factor XI is comprised of two identical polypeptide chains linked by disulfide bonds, which yield a single band at 80,000 upon reduction with 2-mercaptoethanol. The specific clotting activity of purified Human Factor XI is 170 - 200 u/mg. In vivo, Factor XI is activated to Factor XIa by Factor Xlla. The resulting Human Factor XIa consists of two heavy and two light chains held together by disulfide bridges. Buffer composition = 4 mM Sodium Acetate-HCl/0.15 M NaCI/pH 5.3 *Extinction Coefficient (1%) = 13.1 Molecular Weight = 160,000 daltons

Price:

0.1 mg $250

IHFXIa 1111a Human Factor XIa

Prepared from homogeneous Human Factor XI using Human Factor Xlla. This Xlla was removed using a corn trypsin inhibitor column. Complete activation is observed on 10% SDS-PAGE gels. Factor XI, through the contact factor pathway cascade, is activated to Factor XIa via Factor Xlla and High Molecular Weight Kininogen. During activation by Factor Xlla and HK, FXI undergoes proteolytic cleavage in which the Mr=80,000 chain reportedly is cleaved to a heavy and light chain of Mr of about 48,000 and 33,000. This Factor XIa is responsible for the activation of Factor IX to Factor IXa. Unlike other examples of activation of vitamin K-dependent blood-clotting proteins, Factor XIa proteolysis of Factor IX does not require membrane surfaces. Buffer composition = 4 mM Sodium Acetate-HCl/0.15 M NaCl/pH 5.3 *Extinction Coefficient (1%) = 13.1 Molecular Weight = 160,000 daltons

Price:

0.1 mg $345
1.0 mg $1,295

IHFXIII 1313- Human Factor XIII (Fibrin Stabilizing Factor)

Human Factor XIII is a tetramer composed of two pairs of chains held together by noncovalent bonds. After activation of the zymogen via Thrombin to its active enzyme form, Factor XIIIa is responsible for catalyzing the formation of covalent bridges between fibrin units to increase the elasticity of the clot network. The resulting cross-linked fibrin is very insoluble and resistant to lysis. Buffer composition =50 mM Tris-HCl/0.1 M NaCl/1 mM EDTA/10 kal inh units/mL Trasylol/20% glycerol/pH 7.5 *Extinction Coefficient (1%)=13.8 Molecular Weight = 320,000 daltons

Price:

0.25 mg $395
1.0 mg $795

IHXII a1212a- Human Factor aXIIa (Activated Hageman Factor)

Human Factor a-XIIa is a serine protease responsible for the activation of Factor XI to XIa in the contact activation system. Human Factor XII and prekallikrein are thought to be involved in a reciprocal activation mechanism in which Factor XIIa activates prekallikrein to kallikrein, which in turn converts Factor XII to XIIa. Factor XIIa activates Factor XI to XIa thereby triggering the Contact Factor cascade. This purified Human Factor a-XIIa has <5% b form. Buffer composition = 4 mM Sodium Acetate-HCl/0.15 M NaCl/pH 5.3 *Extinction Coefficient (1%) = 14.1 Molecular Weight = 80,000 daltons

Price:

0.5 mg $395
1.0 mg $595

IHPK 1302- Human Prekallikrein

Purified from fresh frozen human plasma. Human Prekallikrein is a single chain gamma globulin glycoprotein that participates in the early phase of contact activation, kinin formation and fibrinolysis. Purified Prekallikrein has a specific clotting activity of approximately 25 u/mg. Prekallikrein is homogeneous as judged by 10% SDS-PAGE gels and shows no reduction upon incubation with 2-mercaptoethanol. Buffer composition = 4 mM Sodium Acetate-HCl/0.15 M NaCl/pH 5.3 *Extinction Coefficient (1%) =11.7 Molecular Weight = 86,000 daltons

Price:

1.0 mg $395
5.0 mg $1,295

IHPKa 1303- Human Kallikrein

Activation of Prekallikrein with Factor a-XIIa produces the enzymatically active Kallikrein. Kallikrein is a serine protease which consists of a heavy chain (Mr 52kD) and light chains (Mr either 36 or 33 kD) linked by disulfide bridges. Kallikrein possesses enzymatic activity toward Factor XII, HK, Plasminogen, Factors XI, IX, and VII, prorenin, and the complement system. After activation, the activating enzyme Factor XIIa is removed by affinity chromatography. Human Kallikrein is homogeneous as judged by 10% SDS-PAGE gels and shows complete reduction upon incubation with 2-mercaptoethanol. Buffer composition = 4 mM Sodium Acetate-HCl/0.15 M NaCl/pH 5.3 *Extinction Coefficient (1%) = 11.7 Molecular Weight = ~86,000 daltons

Price:

1.0 mg $495
5.0 mg $1,495

IHK 1300- Single Chain High Molecular Weight Kininogen

High Molecular Weight Kininogen (HK) is a nonenzymatic cofactor of the contact activation system. HK is thought to have two functions in the contact activation system. First, HK links prekallikrein to a negatively charged surface thereby allowing activation to kallikrein by surface bound Factor a-Xlla. HK also forms a complex with Factor XI and accelerates its activation to XIa by a-Xlla. Also HK serves as a source of Bradykinin, a potent Vasoactive peptide important in Hypotension studies. Buffer composition = 4 mM Sodium Acetate-HCl/0.15 M NaCl/pH 5.3 *Extinction Coefficient (1%) = 7.01 Molecular Weight = 120,000 daltons

Price:

1.0 mg $295
5.0 mg $1,095

I2HK- Two Chain High Molecular Weight Kininogen

This is prepared by Kallikrein digestion of Kininogen which is then re-purified to remove traces of Kallikrein. Buffer composition = 4 mM Sodium Acetate-HCl/0.15 M NaCl/pH 5.3 *Extinction Coefficient (1%) = 7.01 Molecular Weight = 110,000 daltons HPg 2001 Human Glu-Plasminogen Purified from fresh frozen human plasma. This protein is homogeneous as judged by 10% SDS-PAGE gels and shows no reduction upon incubation with 2-mercaptoethanol. No Plasmin activity is detected using the chromogenic substrate S-2251. Plasminogen is activated to the serine protease plasmin via urokinase, streptokinase or tissue plasminogen activator. Buffer composition = 50 mM Tris-HCl/0.1 M NaCl /pH 7.4 *Extinction Coefficient (1%) = 17.0 Molecular Weight = 90,000 daltons

Price:

1.0 mg $295
5.0 mg $1,095

IHAT- Human Antithrombin

Purified from fresh frozen human plasma. AT is a single-chain glycoprotein which is considered to be the main physiological inhibitor of thrombin and Factor Xa. In the presence of heparin, the inhibition of thrombin and factor Xa is enhanced from 300 to 1,000 fold. AT is a single band on 10% SDS-PAGE gels and shows no reduction upon incubation with 2-mercaptoethanol. Buffer composition= 20 mM Tris-HCl/0.1 M Citric Acid/0.15 M NaCl/pH 8.3 *Extinction Coefficient (1%) = 6.5 Molecular Weight = 58,000 daltons

Price:

1.0 mg $195
5.0 mg $495

IHCII- Human Heparin CoFactor II (HCII)

Prepared from fresh frozen human plasma. Heparin Cofactor II is a single chain glycoprotein with a MW of 65,600 daltons. HCII is a specific inhibitor of thrombin with increased activity in the presence of heparin. Heparin Cofactor II is homogeneous as judged by 10% SDS-PAGE gels and the activity is measured by the ability to inhibit thrombin in the presence of saturating concentrations of heparin. Buffer =20 mM Tris-HCl/0.15 M NaCl/pH 7.3, containing 0.02% NaN3 (w/v) *Extinction Coefficient (1%) = 9.1 Molecular Weight = 65,600 daltons

Price:

0.25 mg $395
0.5 mg $595
1.0 mg $995

IGP2b3a- Human GPIIbIIIa

Platelet membrane glycoproteins are involved in platelet adhesion and aggregation. Glycoproteins IIb and IIIa (GPIIb and GPIIIa) constitute the fibrinogen receptor and are required for platelet aggregation. GPIIbIIIa migrates on gels as follows: GPIIb 136,000 non-reduced and 125,000 reduced. GPIIIa is 97,000 non-reduced and 108,000 reduced. Protein concentration is determined by Bradford method. Buffer Composition = 20 mM Tris-HCl/0.1 M NaCl/0.1% Triton X-100/1 mM CaCl2/0.05 NaN3/50% glycerol/ pH 7.4 *Extinction Coefficient (1%) = 9.1

Price:

1.0 mg $295

IRVV-X- Russels Viper Venom

Price:

0.1 mg $95
1.0 mg $395

IARI- Angiostatin

Price:

0.5 mg $495
1.0 mg $695

Antisera

We offer antisera to seven of our human antigens; Human Protein C, Human Protein S, Human Factor II, Human Factor IX, Human factor X, Human Factor XII, and Human Antithrombin. These antisera are developed using rabbits as the host. These antisera should be stored frozen and repeated freezing and thawing should be avoided.