Intravitreal injection of mesenchymal stem cells evokes retinal vascular damage in rats
Posted by Corey Marcath on
Innovative Grade US Origin Rat Sprague Dawley Serum from Innovative Research was used in the following study:
Intravitreal injection of mesenchymal stem cells evokes retinal vascular damage in rats
Hongpeng Huang, Matthias Kolibabka, Rachana Eshwaran, Anupriya Chatterjee, Andrea Schlotterer, Hélène Willer, Karen Bieback, Hans-Peter Hammes, and Yuxi Feng
The FASEB Journal
5 Nov 2019
“…The aim of this study is to investigate the vascular outcome after intravitreal mesenchymal stem cell (MSC) administration in rats without or with damage to the neurovascular unit [transgenic (TGR) rats]. Male Sprague-Dawley (SD) and TGR rats received an intravitreal injection of 2 × 104 rat bone marrow–derived MSCs (BMSCs) or human adipose-derived stem cells (ASCs) at postnatal d 30. After 4 wk, vasculature, neuronal function, and gene expression in the retinas were evaluated using retinal morphometry, electroretinography, immunofluorescence, Western blot, and quantitative PCR. Intravitreal administration of rat BMSCs and human ASCs in both SD and TGR eyes induced cataract, loss of pericytes, and increased formation of acellular capillaries. BMSCs remained in the vitreous cavity and did not migrate into the retinas. Intravitreal administration of BMSCs impacted retinal neuronal function in neither SD nor TGR rats. Retinal glial activation, elevation of IL-1β, C3, arginase 1, and heat shock protein 90 were detected in BMSC-injected SD rats. …Pelleted cells were resuspended in DMEM (Lonza, Basel, Switzerland) supplemented with 10% rat serum (Innovative Research, Novi, MI, USA)…”
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