Complement-dependent bystander injury to neurons in AQP4-IgG seropositive neuromyelitis optica

Human Complement from Innovative Research was used in the following study:

Complement-dependent bystander injury to neurons in AQP4-IgG seropositive neuromyelitis optica

Tianjiao Duan, Alex J. Smith and Alan S. Verkman

Journal of Neuroinflammation, October 2018

"... Specified concentrations of AQP4-IgG (or control human IgG, Thermo Fisher Scientific, Rockford, IL) and human complement (Innovative Research, Novi, MI) were added in Hank’s buffer, and cells were incubated at 37 °C for specified times. In some experiments, cells were exposed to serum of an AQP4-IgG seropositive NMO patient who met the revised diagnostic criteria for clinical disease. A fixable dead-cell stain (amine-reactive dye, Invitrogen, Eugene, OR) at 1:1000 dilution was added 30 min prior to cell fixation. In some experiments, C1q- or C6-deficient human complement (Innovative Research, Novi, MI) was used instead of normal complement ..."

Related products available from Innovative Research also include:

• Single Donor Human Complement Serum
• Pooled Human Complement Serum
• Pooled Human Complement Plasma
• Complement C4 Deficient Human Plasma Lyophilized
• Human IgG Fractionated Purified
• Human Complement Component 1q (C1q) ELISA Kit
• Human Complement Component 6 (C6) ELISA Kit