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Anhydrotrypsin

Treatment of trypsin with phenylmethylsulfonyl fluoride (PMSF) followed by a strong base leads to the conversion of Serine 195 to dehydroalanine at the active site (1). Alpha and beta forms of anhydrotrypsin are separated by immobilized soybean trypsin inhibitor. The activity of anhydrotrypsin is greatly reduced compared to native trypsin as quantified by chromogenic substrate (2). Anhydrotrypsin provided by Innovative Research is the single chain beta-form prepared from Type XIII bovine trypsin. It has a MW of 23.3 kDa and an extinction coefficient of 1.54 mg-1 ml-1.

References 1. Ako, H. et al. (1972) Biochem Biophys Res Commun. 47:1402-1407. 2. Olson, S.T. et al. (1995) J. Biol. Chem. 270:30007-30017.

IANHT - Anhydrotrypsin

0.1 mg - $395

IANHT - Immobilized Anhydrotrypsin

Immobilized anhydrotrypsin selectively adsorbs peptides with Arg, Lys or AECys (S-aminoethyl cysteine) residues at the C-terminus under weak acidic conditions (1). Strongly acidic conditions are used to elute the bound peptides. Peptides with these amino acids at the N-terminus or internally, as well as free amino acids are not bound. Serine protease inhibitors such as ecotin and PAI-1 may also be purified on this resin (2). May be used repeatedly.

References 1. Kumazaki, T. et al. (1987) J. Biochem. (Tokyo) 102:1539-1546. 2. Blouse, G. E. et al. (2003) Biochemistry 42:12260-12272.

1.0 ml - $595

10 ml - $3900

46430 Peary Court
Novi, MI 48377

Phone: (248) 896-0145 Fax: (248) 896-0148