Bovine Coagulation Proteins

IBPC 1003- Bovine Protein C

Purified from fresh citrated bovine plasma using a combination of salt precipitations and column chromatography, this protein is a single band on 10% SDS-PAGE gels and shows total reduction upon incubation with 2-mercaptoethanol. No other coagulation factors are detected by functional assay. Protein C is activated to the serine protease, Activated Protein C (APC), by a -thrombin or the complex of a -thrombin/thrombomodulin. This APC is a potent anticoagulant through the selective inactivation of Factors Va and VIlla. Buffer composition = 20 mM Tris-HCl/0.1 M NaCl/ 1 mM Benzamidine/pH 7.4 *Extinction Coefficient (1%) = 13.7 Molecular Weight = 54,300 daltons

0.1 mg $195.00
1.0 mg $350.00

IBPCa- Bovine Activated Protein C

Activated Protein C (APC) is a serine protease derived from the two chain vitamin K dependent zymogen, Protein C. APC inhibits blood coagulation through the selective inactivation of the cofactors Va and VIIIa. APC is prepared from homogeneous protein C by activation with purified thrombin. This thrombin is removed after activation by ion exchange chromatography. Activated Protein C is homogeneous on 10% SDS-PAGE gels and shows complete reduction upon incubation with 2-mercaptoethanol. Buffer composition = 20 mM Tris-HCl/0.1 M NaCl/ pH 7.4 *Extinction Coefficient (1%) = 13.7 Molecular Weight = 52,650 daltons

0.1 mg $195.00
1.0 mg $395.00

IBT 1002- Bovine Thrombin

Prepared from freshly collected bovine plasma.

<3000 units $95.00 per 1,000 units
>3000 units $75.00 per 1,000 units

IBP 1004- Bovine Prothrombin (Factor II)

Prepared from freshly collected bovine plasma, bovine Prothrombin is a glycoprotein of molecular weight 70,000 and consists of a single polypeptide chain. Activation of Prothrombin by Factor Xa yields the serine protease Thrombin. Prothrombin is homogeneous as judged by 10% SDS-PAGE gels and shows no reduction upon incubation with 2-mercaptoethanol. Buffer composition = 20 mM Tris-HCl/0.1 M NaCl/ 1 mM Benzamidine/pH 7.4 *Extinction Coefficient (1%) =14.4 One unit = 100 µg Molecular Weight =70,000 daltons

100 units (10 mg) $195.00
1000 units (100 mg) $895.00

IBP GD- Bovine Prothrombin (Gla-domainless)

Prepared from freshly collected bovine plasma, bovine Prothrombin is a glycoprotein of molecular weight 70,000 and consists of a single polypeptide chain.

1.0 mg $95.00

IBFV/Va- Bovine Factor V/Va

Purified from freshly collected bovine plasma using a combination of salt precipitations and column chromatography, this protein is a mixture of Factor V/Va and intermediate activation products as judged by 4-20% gradient gels. Bovine Factor Va functions as a cofactor with the serine protease Factor Xa in the activation of prothrombin. Buffer composition = 20 mM HEPES-HCl/0.15 M NaCl/pH 7.4/50% glycerol *Extinction Coefficient (1%) = 9.6/ 1.74 One unit = 10 µg Molecular Weight = 330,000; 94,000; 74,000; 71,000; 31,000 daltons Concentration determined by BCA

100 units (1 mg) $245.00
500 units (5 mg) $795.00

IBFIX 1005- Bovine Factor IX (Christmas Factor)

Prepared from freshly collected bovine plasma, this protein is a single band on 10% SDS-PAGE gels and shows no reduction upon incubation with 2-mercaptoethanol. No other coagulation factors are detected by functional assay. Activated Factor IX via factor Xla or the factor VIIa/tissue factor/phospholipid complex, is responsible for the activation of Factor X to Xa. Buffer composition = 20 mM Tris-HCl/0.1 M NaCl/1 mM Benzamidine/pH 7.4 *Extinction Coefficient (1%) = 14.9 One unit = 5 µg Molecular Weight = 55,400 daltons

100 units (0.5 mg) $195.00
500 units (2.5 mg) $795.00

IBFIXa 1070- Bovine Factor IXaa

Prepared from homogeneous Bovine Factor IX by activation with Russel's Viper Venom, this RVV-X is removed after activation. The RVV-X is responsible for converting Factor IX to an enzyme by cleavage of an internal Arg-Val peptide bond. The Factor IXa produced by RVV-X cleavage possesses a molecular weight identical to that of Factor IX but shows reduction upon incubation with 2-mercaptoethanol. This form of Factor IXa has about 50% of the coagulant activity of Factor IXab. Buffer composition = 20 mM Tris-HCl/0.1 M NaCl/pH 7.4 *Extinction Coefficient (1%) = 14.9 One unit = 5 µg Molecular Weight = 55,400 daltons

100 units (0.5 mg) $395.00
500 units (2.5 mg) $1,195.00

IBFIXa 1080- Bovine Factor IXa

Prepared from homogeneous Bovine Factor IX by activation with Bovine Factor Xla, this Bovine Factor Xla is removed after activation. Complete activation is observed on 10% SDS-PAGE gels. The Factor Xla activates FIX in a two-step reaction. In the first step, an internal Arg-Ala bond is cleaved, and in the second step, an Arg-Val bond is cleaved. The second cleavage leads to the liberation of an activation peptide from the NH2-terminal portion of the heavy chain to produce factor IXab. Buffer composition = 20 mM Tris-HCl/0.1 M NaCl/pH 7.4 *Extinction Coefficient (1%) = 14.3 One unit = 5 µg Molecular Weight = 43,900 daltons

100 units (0.5 mg) $395.00
500 units (2.5 mg) $1,195.00

IBFX 1006- Bovine Factor X (Stuart Factor)

Prepared from freshly collected Bovine plasma, this protein is a single band on 10% SDS-PAGE gels and shows total reduction upon incubation with 2-mercaptoethanol. No other coagulation factors are detected by functional assay. Bovine Factor X, once activated, via the contact factor pathway or tissue factor pathway, is responsible for the conversion of Prothrombin to Thrombin. Buffer composition = 20 mM Tris-HCl/0.1 M NaCl/ 1 mM Benzamidine/pH 7.4 *Extinction Coefficient (1%) = 12.5 One unit = 10 µg Molecular Weight = 55,000 daltons

100 units (1 mg) $350.00
500 units (5 mg) $1,150.00

IBFXA- Bovine Factor Xa Bovine

Factor Xa is prepared from homogeneous Bovine Factor X by activation with Russels' Viper Venom. This RVV-X is removed after activation. Complete activation is observed on 10% SDS-PAGE gels. Factor Xa, as part of the prothrombinase complex along with the cofactor Va, phospholipids and calcium ions, catalyzes the rapid conversion of prothrombin to thrombin. Buffer composition = 20 mM Tris-HCl/0.7 M NaCl/pH 7.4 *Extinction Coefficient (1%) = 12.4 One unit = 10 µg Molecular Weight = 45,300 daltons

100 units (1 mg) $350.00
1000 units (10 mg) $1,995.00

IBFXIa- Bovine Factor XIa Bovine

Factor Xla is purified from freshly collected Bovine Plasma using a combination of salt precipitations and activation on a negative surface. This Factor Xla is a potent activator of both human and Bovine Factor IX to their active form Factor IXab. This BXla is a doublet on 10% SDS-PAGE gels and shows complete reduction upon incubation with 2-mercaptoethanol. Buffer composition = 50 mM Tris-HCl/0.1 M NaCl/1 mM Benzamidine/pH 8.0 Protein concentration is determined by BCA BPG Bovine Plasminogen Purified from freshly collected bovine plasma, this protein is homogeneous as judged by 10% SDS-PAGE gels and shows no reduction upon incubation with 2-mercaptoethanol. No Plasmin activity was detected using the chromogenic substrate S-2251. Plasminogen is activated to the serine protease plasmin via urokinase, streptokinase or tissue plasminogen activator. Buffer composition = 50 mM Tris-HCl/0.1 M NaCl /pH 7.4 *Extinction Coefficient (1%) = 16.1 Molecular Weight = 90,000 - 94,000 daltons

1 mg $195.00

IBAT- Bovine Antithrombin

Purified from freshly collected bovine plasma, BAT is a single-chain glycoprotein which is considered to be the main physiological inhibitor of thrombin and Factor Xa. BAT is a single band on 10% SDS-PAGE gels and shows no reduction upon incubation with 2-mercaptoethanol. Buffer composition = 20 mM Tris-HCl/0.1M Sodium Citrate/0.15 M NaCl/pH 8.3 *Extinction Coefficient (1%) = 6.5 Molecular Weight = 58,000 daltons BFIB1 Fibrinogen, Plasminogen Depleted (Factor I) Purified from freshly collected bovine plasma, this protein is homogeneous as judged by 4-20% gradient gel electrophoresis and is greater than 95% clottable. No other coagulation factors are detected by functional assay. Conversion of soluble fibrinogen to the insoluble clot-forming fibrin is the terminal stage of (blood) coagulation. Buffer composition = 10 mM Phosphate/20 mM Citrate/0.15 M NaCl/pH 7.4 *Extinction Coefficient (1%) = 15.1 Molecular Weight = 330,000 daltons

1 mg $195.00
5 mg $295.00